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OBSERVE: After the coupling of the primary C-terminal amino acid of all peptides to the amino group of the membrane, the amino teams between the spots and likewise among the amino groups inside the spot areas do not kind bonds with the amino acids. BE AWARE: This avoids the coupling of amino acids in the additional cycles with the membrane instead of the growing peptide chain. Ensure that all the purification buffers are at four °C and perform the protein purification in the cold room to keep away from protein denaturation.
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