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This pore is rimmed with a number of structurally conserved carbonyl oxygens as well as the hydroxyl group of the invariant tyrosine from the carboxyl terminus of the SET area (Determine 1 ). These carbonyl and hydroxyl oxygens have been proposed to facilitate the transfer of the methyl group during catalysis 16 , 21 , 24 In addition, tyrosine residues within the lysine-binding clefts of SET7/9 (Tyr245 and Tyr305) and DIM-5 (Tyr178) hydrogen-bond to the lysine ε-amino group, aligning it for a methyltransfer with AdoMet (Determine 4b,c ). Mutation of Tyr245 or Tyr305 in SET7/9 (Figure 4b ) alters its specificity from an H3 K4 mono-methylase to a tri- and di-methylase, respectively sixteen , 21 , whereas an Phe281Tyr mutation in the lysine-binding pocket of DIM-5 (Determine 4c ) converts this protein to an H3 K9 mono- or di-methylase sixteen These mutations exemplify the F/Y swap (Determine 1 ) that establishes SET-domain product specificities.
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Unbound peptides are removed via washing, and the captured PTM-containing peptides are eluted with dilute acid. Other Fish Excessive in Lysine (%RDI per 3oz, cooked): Salmon (100%), Snapper (ninety eight%), Mackerel (97%), Halibut (ninety four%), Pollock (ninety three%), Sea Bass (88%), Haddock (86%), and Cod (85%). Different Soya Meals Excessive in Lysine (%RDI per ounce): [Dried Frozen](https://drtuyet.com/tai-sao-nen-kham-tien-hon-nhan/) Tofu (Koyadofu) (42%), Soy Flour (31%), Soy Chips (22%), Fried Tofu, Natto (Fermented Soybeans) & Boiled Soybeans (15%), and Tempeh (12%).
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